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Western blot(SDS PAGE) analysis of extracts from PC-3 cells.Using Paxillin Rabbit mAb IgG [2N96] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PXN,PAXI_HUMAN,Paxillin
UniProtID :P49023
MASS(da) :64,505
MW(kDa) :60kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Paxillin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC-3 cells
Function : Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Subcellular locationi :Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.