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Western blot(SDS PAGE) analysis of extracts from Mouse liver tissue lysate.Using PEDF/SERPINF1 Rabbit mAb IgG [3Z59] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SERPINF1,PEDF,PIG35,PEDF_HUMAN,Pigment epithelium-derived factor
UniProtID :P36955
MASS(da) :46,312
MW(kDa) :46kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PEDF/SERPINF1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse liver tissue lysate
Function : Neurotrophic protein,induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity..
Tissue specificity :Retinal pigment epithelial cells and blood plasma..
Subcellular locationi :Secreted. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.