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Western blot(SDS PAGE) analysis of extracts from Mouse skeletal muscle tissue lysate.Using PFKM Rabbit mAb IgG [8R22] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PFKM,PFKX,PFKAM_HUMAN,ATP-dependent 6-phosphofructokinase, muscle type
UniProtID :P08237
MASS(da) :85,183
MW(kDa) :81kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PFKM.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse skeletal muscle tissue lysate
Function : Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.