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PGAM1 Rabbit mAb[42A2]Cat NO.: A86300

Western blot(SDS PAGE) analysis of extracts from 293T cells.Using PGAM1 Rabbit mAb IgG [42A2] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PGAM1,PGAMA,CDABP0006,PGAM1_HUMAN,Phosphoglycerate mutase 1

UniProtID :P18669

MASS(da) :28,804

MW(kDa) :28KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PGAM1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cells

Function : Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglyceratea crucial step in glycolysis, by using 2,3-bisphosphoglycerate (PubMed:23653202). Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate (PubMed:23653202)..

Tissue specificity :Expressed in the liver and brain. Not found in the muscle..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.