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Western blot(SDS PAGE) analysis of extracts from Rat brain tissue lysate.Using PIK3R3/p55PIK Rabbit mAb IgG [306Q] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PIK3R3,P55G_HUMAN,Phosphatidylinositol 3-kinase regulatory subunit gamma
UniProtID :Q92569
MASS(da) :54,448
MW(kDa) :54kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PIK3R3/p55PIK.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat brain tissue lysate
Function : Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
Tissue specificity :Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.