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Western blot(SDS PAGE) analysis of extracts from Raji cells.Using PLC gamma 2 (PLCG2) Rabbit mAb IgG [WD87] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PLCG2,PLCG2_HUMAN,1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2
UniProtID :P16885
MASS(da) :147,870
MW(kDa) :148kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PLC gamma 2 (PLCG2).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cells
Function : The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.