* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from U-251MG cells.Using Profilin1 Rabbit mAb IgG [M7W5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PFN1,PROF1_HUMAN,Profilin-1
UniProtID :P07737
MASS(da) :15,054
MW(kDa) :15kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Profilin1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-251MG cells
Function : Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR..
Tissue specificity :Expressed in epididymis (at protein level)..
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.