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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using PSA/KLK3 Rabbit mAb IgG [UZ70] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KLK3,APS,KLK3_HUMAN,Prostate-specific antigen
UniProtID :P07288
MASS(da) :28,741
MW(kDa) :30KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human PSA/KLK3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.