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Rap1GAP Rabbit mAb[AF82]Cat NO.: A64935

Western blot(SDS PAGE) analysis of extracts from BxPC-3 cells.Using Rap1GAP Rabbit mAb IgG [AF82] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :RAP1GAP,KIAA0474,RAP1GA1,RPGP1_HUMAN,Rap1 GTPase-activating protein 1

UniProtID :P47736

MASS(da) :73,361

MW(kDa) :95kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Rap1GAP.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :BxPC-3 cells

Function : GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state..

Tissue specificity :Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues..

Subcellular locationi :Golgi apparatus membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.