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Western blot(SDS PAGE) analysis of extracts from BxPC-3 cells.Using Rap1GAP Rabbit mAb IgG [AF82] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAP1GAP,KIAA0474,RAP1GA1,RPGP1_HUMAN,Rap1 GTPase-activating protein 1
UniProtID :P47736
MASS(da) :73,361
MW(kDa) :95kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Rap1GAP.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :BxPC-3 cells
Function : GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state..
Tissue specificity :Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues..
Subcellular locationi :Golgi apparatus membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.