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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using RBM3 Rabbit mAb IgG [W28O] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RBM3,RNPL,RBM3_HUMAN,RNA-binding protein 3
UniProtID :P98179
MASS(da) :17,170
MW(kDa) :17KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RBM3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity)..
Subcellular locationi :Nucleus. Cytoplasm. Cell projection, dendrite.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.