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RNF14 Rabbit mAb[V1E1]Cat NO.: A92193

Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cells.Using RNF14 Rabbit mAb IgG [V1E1] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :RNF14,ARA54,HRIHFB2038,RNF14_HUMAN,E3 ubiquitin-protein ligase RNF14

UniProtID :Q9UBS8

MASS(da) :53,837

MW(kDa) :54kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RNF14.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :SH-SY5Y cells

Function : Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription..

Tissue specificity :Widely expressed.

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.