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Western blot(SDS PAGE) analysis of extracts from SW620 cells.Using RUNX1 Rabbit mAb IgG [UFC0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RUNX1,AML1,CBFA2,RUNX1_HUMAN,Runt-related transcription factor 1
UniProtID :Q01196
MASS(da) :48,737
MW(kDa) :55KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human RUNX1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SW620 cells
Function : Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5
Tissue specificity :Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.