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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Sec23A Rabbit mAb IgG [AS7L] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SEC23A,SC23A_HUMAN,Protein transport protein Sec23A
UniProtID :Q15436
MASS(da) :86,161
MW(kDa) :86kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Sec23A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex. Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane (By similarity)..
Tissue specificity :Ubiquitously expressed..
Subcellular locationi :Cytoplasmic vesicle, COPII-coated vesicle membrane,Peripheral membrane protein,Cytoplasmic side. Endoplasmic reticulum membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.