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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Sec61A Rabbit mAb IgG [6LV1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SEC61A2,S61A2_HUMAN,Protein transport protein Sec61 subunit alpha isoform 2
UniProtID :Q9H9S3
MASS(da) :52,248
MW(kDa) :52kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Sec61A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins. Found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins (By similarity)..
Subcellular locationi :Endoplasmic reticulum membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.