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Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using Smad5 Rabbit mAb IgG [GTP7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SMAD5,MADH5,SMAD5_HUMAN,Mothers against decapentaplegic homolog 5
UniProtID :Q99717
MASS(da) :52,258
MW(kDa) :60kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Smad5.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K-562 cells
Function : Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Tissue specificity :Ubiquitous.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.