ACSL1 Rabbit mAb [5Z43]Cat NO.: A39248

Protein names ：ACSL1,FACL1,FACL2,LACS,LACS1,LACS2,ACSL1_HUMAN,Long-chain-fatty-acid--CoA ligase 1

UniProtID ：P33121

MASS(da) ：77,943

MW(kDa) ：78 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human ACSL1

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：293T cells

Function ： Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:24269233, PubMed:22633490, PubMed:21242590). Preferentially uses palmitoleate, oleate and linoleate (PubMed:24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity)..

Tissue specificity ：Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas..

Subcellular locationi ：Mitochondrion outer membrane,Single-pass type III membrane protein. Peroxisome membrane,Single-pass type III membrane protein. Microsome membrane,Single-pass type III membrane protein. Endoplasmic reticulum membrane,Single-pass type III membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.