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Western blot(SDS PAGE) analysis of extracts from U-251MG cells.Using Syndecan 3 Rabbit mAb IgG [S2P2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SDC3,KIAA0468,SDC3_HUMAN,Syndecan-3
UniProtID :O75056
MASS(da) :45,497
MW(kDa) :70KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Syndecan 3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-251MG cells
Function : Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism..
Tissue specificity :Expressed in the nervous system, the adrenal gland, and the spleen..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.