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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using TIMM50 Rabbit mAb IgG [VMD0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TIMM50,TIM50,PRO1512,TIM50_HUMAN,Mitochondrial import inner membrane translocase subunit TIM50
UniProtID :Q3ZCQ8
MASS(da) :39,646
MW(kDa) :38kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TIMM50.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro,however such activity may not be relevant in vivo.., [Isoform 2]: May participate in the release of snRNPs and SMN from the Cajal body..
Tissue specificity :Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level)..
Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein.,[Isoform 2]: Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.