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TIMM50 Rabbit mAb[VMD0]Cat NO.: A15641

Western blot(SDS PAGE) analysis of extracts from 293T cells.Using TIMM50 Rabbit mAb IgG [VMD0] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TIMM50,TIM50,PRO1512,TIM50_HUMAN,Mitochondrial import inner membrane translocase subunit TIM50

UniProtID :Q3ZCQ8

MASS(da) :39,646

MW(kDa) :38kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TIMM50.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cells

Function : Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro,however such activity may not be relevant in vivo.., [Isoform 2]: May participate in the release of snRNPs and SMN from the Cajal body..

Tissue specificity :Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level)..

Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein.,[Isoform 2]: Nucleus speckle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.