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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using TSFM Rabbit mAb IgG [K5LQ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TSFM,EFTS_HUMAN,Elongation factor Ts, mitochondrial
UniProtID :P43897
MASS(da) :35,391
MW(kDa) :35KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human TSFM.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome..
Tissue specificity :Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney.
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.