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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using USO1 Rabbit mAb IgG [50X4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :USO1,VDP,USO1_HUMAN,General vesicular transport factor p115
UniProtID :O60763
MASS(da) :107,895
MW(kDa) :115KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human USO1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : General vesicular transport factor required for intercisternal transport in the Golgi stack,it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity..
Subcellular locationi :Cytoplasm, cytosol. Golgi apparatus membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.