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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using ZHX2 Rabbit mAb IgG [Y914] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ZHX2,AFR1,KIAA0854,RAF,ZHX2_HUMAN,Zinc fingers and homeoboxes protein 2
UniProtID :Q9Y6X8
MASS(da) :92,307
MW(kDa) :110KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ZHX2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Acts as a transcriptional repressor (PubMed:12741956). Represses the promoter activity of the CDC25C gene stimulated by NFYA (PubMed:12741956). May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity)..
Tissue specificity :Ubiquitously expressed. Expressed in podocytes..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.