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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using 14-3-3E Mouse mAb IgG [W515] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :YWHAE,1433E_HUMAN,14-3-3 protein epsilon
UniProtID :P62258
MASS(da) :29,174
MW(kDa) :30kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human 14-3-3E.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326)..
Subcellular locationi :Nucleus. Cytoplasm. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.