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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ACAT2 Mouse mAb IgG [SRBC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACAT2,ACTL,THIC_HUMAN,Acetyl-CoA acetyltransferase, cytosolic
UniProtID :Q9BWD1
MASS(da) :41,351
MW(kDa) :42kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ACAT2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Involved in the biosynthetic pathway of cholesterol..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.