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Western blot(SDS PAGE) analysis of extracts from human heart tissue.Using ACTC1-specific Mouse mAb IgG [O5X5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ACTC1,ACTC,ACTC_HUMAN,Actin, alpha cardiac muscle 1 [Cleaved into: Actin, alpha cardiac muscle 1, intermediate form]
UniProtID :P68032
MASS(da) :42,019
MW(kDa) :42kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ACTC1-specific.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human heart tissue
Function : Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular locationi :Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.