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Western blot(SDS PAGE) analysis of extracts from L02 cells.Using ADH1B Mouse mAb IgG [T26K] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ADH1B,ADH2,ADH1B_HUMAN,All-trans-retinol dehydrogenase [NAD] ADH1B
UniProtID :P00325
MASS(da) :39,835
MW(kDa) :40kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ADH1B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :L02 cells
Function : Catalyzes the NAD-dependent oxidation of all-trans-retinol and its derivatives such as all-trans-4-hydroxyretinol and may participate in retinoid metabolism (PubMed:15369820, PubMed:16787387). In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and its derivatives such as all-trans-4-oxoretinal (PubMed:15369820, PubMed:16787387). Catalyzes in the oxidative direction with higher efficiency (PubMed:16787387). Has the same affinity for all-trans-4-hydroxyretinol and all-trans-4-oxoretinal (PubMed:15369820)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.