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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using ALDH9A1 Mouse mAb IgG [BN6V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ALDH9A1,ALDH4,ALDH7,ALDH9,AL9A1_HUMAN,4-trimethylaminobutyraldehyde dehydrogenase [Cleaved into: 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed]
UniProtID :P49189
MASS(da) :53,802
MW(kDa) :46kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ALDH9A1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency (in vitro). Can catalyze the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, but with low efficiency..
Tissue specificity :Detected in brain (at protein level) (PubMed:8645224). High expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain (PubMed:8786138). Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.