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Western blot(SDS PAGE) analysis of extracts from mouse eye tissue.Using Alpha B Crystallin Mouse mAb IgG [QQSH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CRYAB,CRYA2,HSPB5,CRYAB_HUMAN,Alpha-crystallin B chain -crystallin)
UniProtID :P02511
MASS(da) :20,159
MW(kDa) :20kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Alpha B Crystallin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse eye tissue
Function : May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity)..
Tissue specificity :Lens as well as other tissues (PubMed:838078, PubMed:2387586). Expressed in myocardial tissue (PubMed:28493373)..
Subcellular locationi :Cytoplasm. Nucleus. Secreted. Lysosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.