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Alpha Sarcoglycan Mouse mAb[M35L]Cat NO.: A62271

Western blot(SDS PAGE) analysis of extracts from human skeletal muscle tissue.Using Alpha Sarcoglycan Mouse mAb IgG [M35L] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SGCA,ADL,DAG2,SGCA_HUMAN,Alpha-sarcoglycan

UniProtID :Q16586

MASS(da) :42,875

MW(kDa) :50kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Alpha Sarcoglycan.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human skeletal muscle tissue

Function : Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.

Tissue specificity :Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.

Subcellular locationi :Cell membrane, sarcolemma,Single-pass type I membrane protein. Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.