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Western blot(SDS PAGE) analysis of extracts from mouse heart tissue.Using ANKRD1 Mouse mAb IgG [GG81] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ANKRD1,C193,CARP,HA1A2,ANKR1_HUMAN,Ankyrin repeat domain-containing protein 1
UniProtID :Q15327
MASS(da) :36,252
MW(kDa) :36kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ANKRD1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse heart tissue
Function : May play an important role in endothelial cell activation. May act as a nuclear transcription factor that negatively regulates the expression of cardiac genes. Induction seems to be correlated with apoptotic cell death in hepatoma cells..
Tissue specificity :Mainly expressed in activated vascular endothelial cells. To a lower extent, also expressed in hepatoma cells..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.