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Western blot(SDS PAGE) analysis of extracts from COLO 320 cells.Using Annexin A4 Mouse mAb IgG [FE4V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ANXA4,ANX4,ANXA4_HUMAN,Annexin A4
UniProtID :P09525
MASS(da) :35,883
MW(kDa) :32kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Annexin A4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :COLO 320 cells
Function : Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis..
Subcellular locationi :Zymogen granule membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.