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ARF5 Mouse mAb[BB99]Cat NO.: A75217

Western blot(SDS PAGE) analysis of extracts from A549 cells.Using ARF5 Mouse mAb IgG [BB99] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ARF5,ARF5_HUMAN,ADP-ribosylation factor 5

UniProtID :P84085

MASS(da) :20,530

MW(kDa) :20kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ARF5.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cells

Function : GTP-binding protein involved in protein trafficking,may modulate vesicle budding and uncoating within the Golgi apparatus.., (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase..

Subcellular locationi :Golgi apparatus. Cytoplasm, perinuclear region. Membrane,Lipid-anchor. Golgi apparatus, trans-Golgi network membrane,Lipid-anchor.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.