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Western blot(SDS PAGE) analysis of extracts from A549 cells.Using ARHGEF16 Mouse mAb IgG [3D0K] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ARHGEF16,EPHEXIN4,NBR,ARHGG_HUMAN,Rho guanine nucleotide exchange factor 16
UniProtID :Q5VV41
MASS(da) :80,105
MW(kDa) :80kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ARHGEF16.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cells
Function : Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.