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BCAT1 Mouse mAb[K0FG]Cat NO.: A63073

Western blot(SDS PAGE) analysis of extracts from Ramos cells.Using BCAT1 Mouse mAb IgG [K0FG] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :BCAT1,BCT1,ECA39,BCAT1_HUMAN,Branched-chain-amino-acid aminotransferase, cytosolic )

UniProtID :P54687

MASS(da) :42,966

MW(kDa) :45kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human BCAT1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Ramos cells

Function : Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Tissue specificity :During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.