BID Mouse mAb[22L4]Cat NO.: A95092

Protein names ：BID,BID_HUMAN,BH3-interacting domain death agonist [Cleaved into: BH3-interacting domain death agonist p15 ,BH3-interacting domain death agonist p13 ,BH3-interacting domain death agonist p11 ]

UniProtID ：P55957

MASS(da) ：21,995

MW(kDa) ：22kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human BID.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity).., [BH3-interacting domain death agonist p15]: Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622).., [Isoform 1]: Induces ICE-like proteases and apoptosis.., [Isoform 2]: Induces ICE-like proteases and apoptosis.., [Isoform 3]: Does not induce apoptosis.., [Isoform 4]: Induces ICE-like proteases and apoptosis..

Tissue specificity ：[Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level)..,[Isoform 3]: Expressed in lung, pancreas and spleen (at protein level)..,[Isoform 4]: Expressed in lung and pancreas (at protein level)..

Subcellular locationi ：Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.