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Calbindin-D28k Mouse mAb[I0ZD]Cat NO.: A70662

Western blot(SDS PAGE) analysis of extracts from PC-12 cells.Using Calbindin-D28k Mouse mAb IgG [I0ZD] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CALB1,CAB27,CALB1_HUMAN,Calbindin

UniProtID :P05937

MASS(da) :30,025

MW(kDa) :28kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Calbindin-D28k.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :PC-12 cells

Function : Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.