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Western blot(SDS PAGE) analysis of extracts from human skeletal muscle tissue.Using Calsequestrin 2 Mouse mAb IgG [E82N] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CASQ2,CASQ2_HUMAN,Calsequestrin-2
UniProtID :O14958
MASS(da) :46,436
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Calsequestrin 2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human skeletal muscle tissue
Function : Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR2,this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats..
Subcellular locationi :Sarcoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.