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Cardiac Troponin I Mouse mAb[1750]Cat NO.: A93857

Western blot(SDS PAGE) analysis of extracts from human heart tissue.Using Cardiac Troponin I Mouse mAb IgG [1750] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TNNI3,TNNC1,TNNI3_HUMAN,Troponin I, cardiac muscle

UniProtID :P19429

MASS(da) :24,008

MW(kDa) :28kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Cardiac Troponin I.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human heart tissue

Function : Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.