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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using CHCHD2 Mouse mAb IgG [089N] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CHCHD2,C7orf17,AAG10,CHCH2_HUMAN,Coiled-coil-helix-coiled-coil-helix domain-containing protein 2
UniProtID :Q9Y6H1
MASS(da) :15,513
MW(kDa) :17kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CHCHD2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Transcription factor. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen) (PubMed:23303788)..
Subcellular locationi :Nucleus. Mitochondrion. Mitochondrion intermembrane space.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.