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CNPase Mouse mAb[85QI]Cat NO.: A55700

Western blot(SDS PAGE) analysis of extracts from U-251 cells.Using CNPase Mouse mAb IgG [85QI] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CNP,CN37_HUMAN,2',3'-cyclic-nucleotide 3'-phosphodiesterase

UniProtID :P09543

MASS(da) :47,579

MW(kDa) :48kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human CNPase.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :U-251 cells

Function : Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates (By similarity). May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin (By similarity)..

Subcellular locationi :Membrane,Lipid-anchor. Melanosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.