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Collagen Type I Mouse mAb[4L77]Cat NO.: A96544

Western blot(SDS PAGE) analysis of extracts from human cervical cancer tissue.Using Collagen Type I Mouse mAb IgG [4L77] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :COL1A1,CO1A1_HUMAN,Collagen alpha-1 chain

UniProtID :P02452

MASS(da) :138,911

MW(kDa) :130kda

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100,
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Collagen Type I.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human cervical cancer tissue

Function : Type I collagen is a member of group I collagen (fibrillar forming collagen).

Tissue specificity :Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Subcellular locationi :Secreted, extracellular space, extracellular matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.