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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using ASF1B Rabbit mAb [ZLOZ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ASF1B,ASF1B_HUMAN,Histone chaperone ASF1B
UniProtID :Q9NVP2
MASS(da) :22,434
MW(kDa) :19 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human ASF1B
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis..
Tissue specificity :Highly expressed in testis and at lower levels in colon, small intestine and thymus..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.