CYB5R3 Mouse mAb[9Z6N]Cat NO.: A80826

Protein names ：CYB5R3,DIA1,NB5R3_HUMAN,NADH-cytochrome b5 reductase 3 [Cleaved into: NADH-cytochrome b5 reductase 3 membrane-bound form,NADH-cytochrome b5 reductase 3 soluble form]

UniProtID ：P00387

MASS(da) ：34,235

MW(kDa) ：33kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human CYB5R3.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： Transfers two electrons from NADH to two molecules of cytochrome b5 through an enzyme-bound FAD. Electrons from the reduced cytochrome b5 are then transferred to various electron acceptors, thereby participating in methemoglobin reduction in red blood cells and in the desaturation and elongation of fatty acids, cholesterol biosynthesis and cytochrome P-450-mediated drug metabolism other tissues..

Tissue specificity ：Isoform 2 is expressed at late stages of erythroid maturation.

Subcellular locationi ：[Isoform 1]: Endoplasmic reticulum membrane,Lipid-anchor,Cytoplasmic side. Mitochondrion outer membrane,Lipid-anchor,Cytoplasmic side.,[Isoform 2]: Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.