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Western blot(SDS PAGE) analysis of extracts from A431 cells.Using Cytokeratin 6A Mouse mAb IgG [X00R] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KRT6A,K6A,KRT6D,K2C6A_HUMAN,Keratin, type II cytoskeletal 6A
UniProtID :P02538
MASS(da) :60,045
MW(kDa) :60kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Cytokeratin 6A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells
Function : Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair..
Tissue specificity :Expressed in the corneal epithelium (at protein level)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.