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Western blot(SDS PAGE) analysis of extracts from HEK-293 cells.Using DDX54 Mouse mAb IgG [572T] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DDX54,DDX54_HUMAN,ATP-dependent RNA helicase DDX54
UniProtID :Q8TDD1
MASS(da) :98,595
MW(kDa) :98kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human DDX54.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293 cells
Function : Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors..
Tissue specificity :Expressed in the fallopian tube, cervix and uterus. Also expressed in the brain..
Subcellular locationi :Nucleus, nucleolus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.