EEF1A1 Mouse mAb[077D]Cat NO.: A66312

Protein names ：EEF1A1,EEF1A,EF1A,LENG7,EF1A1_HUMAN,Elongation factor 1-alpha 1

UniProtID ：P68104

MASS(da) ：50,141

MW(kDa) ：49kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Mouse

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100,
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide of human EEF1A1.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cells

Function ： This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (PubMed:17177976).., (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection..

Tissue specificity ：Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

Subcellular locationi ：Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.