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EPO Mouse mAb[Q53H]Cat NO.: A43140

Western blot(SDS PAGE) analysis of extracts from recombinant hEPO.Using EPO Mouse mAb IgG [Q53H] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :EPO,EPO_HUMAN,Erythropoietin

UniProtID :P01588

MASS(da) :21,307

MW(kDa) :21kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human EPO.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :recombinant hEPO

Function : Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3..

Tissue specificity :Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Subcellular locationi :Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.