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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using ERP44 Mouse mAb IgG [QD7P] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ERP44,KIAA0573,TXNDC4,UNQ532/PRO1075,ERP44_HUMAN,Endoplasmic reticulum resident protein 44
UniProtID :Q9BS26
MASS(da) :46,971
MW(kDa) :47kda
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ERP44.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum..
Subcellular locationi :Endoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.