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Western blot(SDS PAGE) analysis of extracts from human plasma tissue.Using Factor X Mouse mAb IgG [RLON] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :F10,FA10_HUMAN,Coagulation factor X [Cleaved into: Factor X light chain,Factor X heavy chain,Activated factor Xa heavy chain]
UniProtID :P00742
MASS(da) :54,732
MW(kDa) :50kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Factor X.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human plasma tissue
Function : Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificity :Plasma,synthesized in the liver..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.