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Flotillin 1 Mouse mAb[8Y3P]Cat NO.: A88859

Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using Flotillin 1 Mouse mAb IgG [8Y3P] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :FLOT1,FLOT1_HUMAN,Flotillin-1

UniProtID :O75955

MASS(da) :47,355

MW(kDa) :47kDa

Form :Liquid

Purification :Protein A purification

Host :Mouse

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Flotillin 1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :LNCaP cells

Function : May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.

Subcellular locationi :Cell membrane,Peripheral membrane protein. Endosome. Membrane, caveola,Peripheral membrane protein. Melanosome. Membrane raft.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.