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Western blot(SDS PAGE) analysis of extracts from LNCaP cells.Using Flotillin 1 Mouse mAb IgG [8Y3P] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FLOT1,FLOT1_HUMAN,Flotillin-1
UniProtID :O75955
MASS(da) :47,355
MW(kDa) :47kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Flotillin 1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :LNCaP cells
Function : May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Subcellular locationi :Cell membrane,Peripheral membrane protein. Endosome. Membrane, caveola,Peripheral membrane protein. Melanosome. Membrane raft.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.