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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using G6PD Mouse mAb IgG [FW2D] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :G6PD,G6PD_HUMAN,Glucose-6-phosphate 1-dehydrogenase
UniProtID :P11413
MASS(da) :59,257
MW(kDa) :60kDa
Form :Liquid
Purification :Protein A purification
Host :Mouse
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human G6PD.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis..
Tissue specificity :Isoform Long is found in lymphoblasts, granulocytes and sperm.
Subcellular locationi :Cytoplasm, cytosol. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.